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dead cells exclusion with draq7  (Miltenyi Biotec)


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    Miltenyi Biotec dead cells exclusion with draq7
    Dead Cells Exclusion With Draq7, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dead+cells+exclusion+with+draq7/DRAQ7+Staining+Solution/bio_rxiv__64898__2026__01__10__698142-519-33-39
    Average 99 stars, based on 18 article reviews
    dead cells exclusion with draq7 - by Bioz Stars, 2026-09
    99/100 stars

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    Staining:

    Article Title: Kidney disease reprograms microbiome-host signaling to promote heart failure
    Article Snippet: T cells were isolated from PBMC using MojoSort Human CD4 T Cell Isolation Kit (Biolegend) with LS columns (Miltenyi) following the manufacturer’s protocol. .. To obtain naïve CD4 cells, the enriched T helper cell fraction was sorted using a BD FACSAria III (BD Bioscience, 70 μm nozzle) by staining for CD4, CD25, CD127, CD45RO (Table S8), and dead cells exclusion with DRAQ7 (1:250, Miltenyi Biotec). .. Naïve CD4 T cells were resuspended in X-VIVO 15 serum-free hematopoietic media (Lonza Bioscience) and plated at 5x10 4 cells per well in anti-CD3-coated (10 μg/mL, BD) 96-well U-bottom plates and 1 μg/mL anti-CD28 (BD).



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